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HiMedia Laboratories middlebrook oadc growth supplement
Middlebrook Oadc Growth Supplement, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/middlebrook+oadc+growth+supplement/middlebrook+oadc+growth+supplement/pm39676255-77-13-17
Average 90 stars, based on 1 article reviews
middlebrook oadc growth supplement - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Derivatives of 3-Aminopyrazine-2-carboxamides: Synthesis, Antimicrobial Evaluation, and in Vitro Cytotoxicity
Article Snippet: Culturing medium was Middlebrook 7H9 broth (Sigma-Aldrich) enriched with 0.4% of glycerol (Sigma-Aldrich) and 10% of Middlebrook OADC growth supplement (Himedia, Mumbai, India).

Article Title: Proteinaceous Secretory Metabolites of Probiotic Human Commensal Enterococcus hirae 20c, E. faecium 12a and L12b as Antiproliferative Agents Against Cancer Cell Lines
Article Snippet: M. smegmatis was grown aerobically at 37°C in 7H9 broth (HiMedia) supplemented with Middlebrook OADC growth supplement (HiMedia) containing bovine serum albumin fraction V and Tween-80.

Article Title: 5-Alkylamino- N -phenylpyrazine-2-carboxamides: Design, Preparation, and Antimycobacterial Evaluation
Article Snippet: Culturing medium was Middlebrook 7H9 broth (Sigma–Aldrich, Steinheim, Germany) enriched with 0.4% of glycerol (Sigma–Aldrich) and 10% of Middlebrook OADC growth supplement (Himedia, Mumbai, India).

Article Title: Derivatives of 3-Aminopyrazine-2-carboxamides: Synthesis, Antimicrobial Evaluation, and in Vitro Cytotoxicity
Article Snippet: Culturing medium was Middlebrook 7H9 broth (Sigma-Aldrich) enriched with 0.4% of glycerol (Sigma-Aldrich) and 10% of Middlebrook OADC growth supplement (Himedia, Mumbai, India).

Article Title: Ureidopyrazine Derivatives: Synthesis and Biological Evaluation as Anti-Infectives and Abiotic Elicitors
Article Snippet: Culturing medium was Middlebrook 7H9 broth (Sigma-Aldrich) enriched with 0.4% of glycerol (Sigma-Aldrich) and 10% of Middlebrook OADC growth supplement (Himedia).

Article Title: N-Pyrazinoyl Substituted Amino Acids as Potential Antimycobacterial Agents—the Synthesis and Biological Evaluation of Enantiomers
Article Snippet: The culturing medium was Middlebrook 7H9 broth (Sigma-Aldrich) with a defined pH of 6.6 ± 0.2 enriched with 0.4% of glycerol (Sigma-Aldrich) and 10% of Middlebrook OADC growth supplement (Himedia, Mumbai, India).

Article Title: Design, Synthesis and Antimicrobial Evaluation of New N -(1-Hydroxy-1,3-dihydrobenzo[ c ][1,2]oxaborol-6-yl)(hetero)aryl-2-carboxamides as Potential Inhibitors of Mycobacterial Leucyl-tRNA Synthetase
Article Snippet: The culture medium was Middlebrook 7H9 broth (Merck, Darmstadt, Germany) enriched with glycerol (Merck, Darmstadt, Germany) and Middlebrook OADC growth supplement (Himedia, Mumbai, India) according to manufacturer instructions.

Cell Culture:

Article Title: Thymol as Biofilm and Efflux Pump Inhibitor: A Dual-Action Approach to Combat Mycobacterium tuberculosis.
Article Snippet: Tuberculosis (TB) remains a significant global health challenge, exacerbated by the emergence of drug‐resistant strains of Mycobacterium tuberculosis (M. tb).. The complex biology of M. tb, particularly its key porins, contributes to its resilience against conventional treatments, highlighting the exploration of innovative therapeutic strategies.. Following with this challenges, the present study investigates the bioactivity properties of phenolic compounds derived from the terpene groups, specifically through Thymol (THY) against M. smegmatis as a surrogated model for M. tb.



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Conventional Bactec MGIT (MGIT tube containing <t>PANTA-OADC</t> antimicrobial enrichment media) and TiKa (MGIT tube containing PANTA-OADC and <t>TiKa</t> <t>supplement</t> B) culture results represented as median time to positivity (d) with interquartile ranges (IQR) for sterile phosphate buffer spiked in duplicate with known amounts (cfu) of A. Mycobacterium bovis SB0121 (10,000–10 cfu) and B. M. tuberculosis H37Rv (10,000–10 cfu), respectively. Prior to inoculation into growth indicator tubes, all spiked samples were either: decontaminated with MycoPrep (treatment 1: MycoPrep MGIT; treatment 2: MycoPrep TiKa), not decontaminated (treatment 3: MGIT control; treatment 4: TiKa control), or decontaminated with KiC agent (treatment 5: KiC MGIT; treatment 6: KiC TiKa). For some data points, the IQRs were shorter than the height of the bar; in these cases, the ranges were not drawn. Using the multiple t -test with Bonferroni–Dunn correction for multiple testing (alpha = 0.01), statistical significance ( p < 0.01) was calculated between treatments for each amount of mycobacteria. Within each amount of the target Mycobacterium , bars with a different superscript are significantly different at p < 0.01.
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Conventional Bactec MGIT (MGIT tube containing PANTA-OADC antimicrobial enrichment media) and TiKa (MGIT tube containing PANTA-OADC and TiKa supplement B) culture results represented as median time to positivity (d) with interquartile ranges (IQR) for sterile phosphate buffer spiked in duplicate with known amounts (cfu) of A. Mycobacterium bovis SB0121 (10,000–10 cfu) and B. M. tuberculosis H37Rv (10,000–10 cfu), respectively. Prior to inoculation into growth indicator tubes, all spiked samples were either: decontaminated with MycoPrep (treatment 1: MycoPrep MGIT; treatment 2: MycoPrep TiKa), not decontaminated (treatment 3: MGIT control; treatment 4: TiKa control), or decontaminated with KiC agent (treatment 5: KiC MGIT; treatment 6: KiC TiKa). For some data points, the IQRs were shorter than the height of the bar; in these cases, the ranges were not drawn. Using the multiple t -test with Bonferroni–Dunn correction for multiple testing (alpha = 0.01), statistical significance ( p < 0.01) was calculated between treatments for each amount of mycobacteria. Within each amount of the target Mycobacterium , bars with a different superscript are significantly different at p < 0.01.

Journal: Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc

Article Title: Improved detection of Mycobacterium tuberculosis and M. bovis in African wildlife samples using cationic peptide decontamination and mycobacterial culture supplementation

doi: 10.1177/10406387211044192

Figure Lengend Snippet: Conventional Bactec MGIT (MGIT tube containing PANTA-OADC antimicrobial enrichment media) and TiKa (MGIT tube containing PANTA-OADC and TiKa supplement B) culture results represented as median time to positivity (d) with interquartile ranges (IQR) for sterile phosphate buffer spiked in duplicate with known amounts (cfu) of A. Mycobacterium bovis SB0121 (10,000–10 cfu) and B. M. tuberculosis H37Rv (10,000–10 cfu), respectively. Prior to inoculation into growth indicator tubes, all spiked samples were either: decontaminated with MycoPrep (treatment 1: MycoPrep MGIT; treatment 2: MycoPrep TiKa), not decontaminated (treatment 3: MGIT control; treatment 4: TiKa control), or decontaminated with KiC agent (treatment 5: KiC MGIT; treatment 6: KiC TiKa). For some data points, the IQRs were shorter than the height of the bar; in these cases, the ranges were not drawn. Using the multiple t -test with Bonferroni–Dunn correction for multiple testing (alpha = 0.01), statistical significance ( p < 0.01) was calculated between treatments for each amount of mycobacteria. Within each amount of the target Mycobacterium , bars with a different superscript are significantly different at p < 0.01.

Article Snippet: M. bovis SB0121 and M. tuberculosis H37Rv stock cultures (Stellenbosch University) were subcultured in separate T25 culture flasks (Thermo Fisher) containing 5 mL of liquid Middlebrook 7H9 broth medium (Merck), 0.05% Tween 80 (Merck), 0.2% glycerol, and 10% Middlebrook oleic acid dextrose citrate (OADC) growth supplement (Merck) at 37°C for 5 d. The optical density (OD) was determined, and cultures diluted to an OD = 0.05 in 30 mL of 7H9 culture medium in T75 culture flasks.

Techniques: